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Investigating a Functionalized Terthiophene Surface for the Detection of Progesterone Using Surface Plasmon Resonance

Marsilea A. Booth 1, Ashton Partridge 2, and Yinqiu Wu 3
1. Digital Sensing Limited, Auckland, New Zealand
2. The University of Auckland, Auckland, New Zealand
3. The New Zealand Institute for Plant & Food Research Limited, Auckland, New Zealand
Abstract—Herein we report a rapid and sensitive surface Plasmon resonance (SPR) biosensor to detect progesterone (P4), a model analyte of small molecules. A terthiophene scaffold with an oligoethylene glycol (OEG) linker is used as an anchor for the P4-ovalbumin (OVA) immobilization on gold to form a stable, regenerable SPR surface. Subsequently, inhibition SPR immunoassays based on this terthiophene/OVA-P4 surface have demonstrated that P4 limit of detection (LOD) in buffer solution was 0.13 ng ml-1. Due to the rapidity of each measurement (less than 5 min per measurement), and the high sensitivity of the assay, we believe the sensing platform may hold great potential for P4 detections.
 
Index Terms—terthiophene, progesterone, surface plasmon resonance, biosensor

Cite: Marsilea A. Booth, Ashton Partridge, and Yinqiu Wu, "Investigating a Functionalized Terthiophene Surface for the Detection of Progesterone Using Surface Plasmon Resonance," International Journal of Electronics and Electrical Engineering, Vol. 4, No. 2, pp. 181-184, April 2016. doi: 10.18178/ijeee.4.2.181-184
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